Dr. Carlos Penedo

Position:
Research Fellow
Research Theme:
Condensed Matter and Photonics
Research Group:
Soft Matter, Statistical Physics and Biophysics
Institution:
St. Andrews
Email address:
jcp10@st-andrews.ac.uk
Website:
http://www.st-andrews.ac.uk/~singlemol/singlemol.html
Telephone number:
+44 (0)1334 463106
Address:
School of Physics & Astronomy, Physical Science Building, North Haugh, St Andrews, KY16 9SS, United Kingdom

Research interests

We are interested in the use and development of novel biophysical techniques to study biomolecular interactions including proteins, DNA and RNA at the level of individual molecules. The advantages of single-molecule detection are many, apart from the fascination of looking at individual biomolecules at work, single-molecule techniques can measure intermediates and follow time-dependent pathways of chemical reactions and folding mechanisms that are difficult or impossible to synchronize at the ensemble level. Thus, single-molecule techniques provide novel insights into how molecules and systems behave, having the advantage that spatial and temporal averaging is avoided, temporal synchronisation is not necessary and novel phenomena, which otherwise are averaged and remain hidden in ensembles, may be discovered. Our aim is to monitor, in real-time, the behaviour of individual biological molecules and complexes, in vitro and in live cells. By combining the dynamic information obtained at the single-molecule or single-complex level with structural and biochemical analyses, we hope to create "molecular movies" of biological processes and from them obtain a deeper understanding of these processes.

http://www.st-andrews.ac.uk/~singlemol/singlemol.html

Research outputs

  1. Real-time observation of conformational switching in single conjugated polymer chains DOI
    Francisco Tenopala Carmona, Stephanie Fronk, Guillermo C. Bazan, Ifor David William Samuel and Juan Carlos Penedo-Esteiro, Science Advances, 4, 2 (2018)
  2. DNA binding and unwinding by Hel308 helicase requires dual functions of a winged helix domain DOI
    Sarah J Northall, Ryan Buckley, Nathan Jones, Juan Carlos Penedo-Esteiro, Panos Soultanas and Edward Bolt, DNA Repair, 57, p. 125-132 (2017)
  3. Transcriptional dynamics DOI
    Daniel A Lafontaine and Juan Carlos Penedo-Esteiro, Methods, 120, p. 1-2 (2017)
  4. An integrated perspective on RNA aptamer ligand-recognition models DOI
    K. McCluskey and J. Carlos Penedo, Physical Chemistry Chemical Physics, 19, 10 , p. 6921-6932 (2017)
  5. High-affinity RNA binding by a hyperthermophilic single-stranded DNA-binding protein DOI
    Michael John Morten, Roland Gamsjaeger, Liza Cubeddu, Ruvini Kariawasam, Jose Ramon Peregrina, Juan Carlos Penedo-Esteiro and Malcolm Frederick White, Extremophiles, 21, 2 , p. 369-379 (2017)
  6. Fluorescence-based strategies to investigate the structure and dynamics of aptamer-ligand complexes DOI
    Daniel Cibran Perez Gonzalez, Daniel A Lafontaine and Juan Carlos Penedo-Esteiro, Frontiers in Chemistry, 4 (2016)
  7. Morphology-specific inhibition of β-amyloid aggregates by 17β-hydroxysteroid dehydrogenase type 10 DOI
    Laura Aitken, Steven David Quinn, D. Cibran Perez-Gonzalez, Ifor David William Samuel, J. Carlos Penedo-Esteiro and Francis James Gunn-Moore, ChemBioChem, 17, 11 , p. 1029-1037 (2016)
  8. Biophysical approaches to gene regulation by riboswitches DOI
    Daniel Cibran Perez Gonzalez, Jonathan P Grondin, Daniel A Lafontaine and Juan Carlos Penedo-Esteiro, Biophysics of Infection, 915, p. 157-190 (2016)
  9. Mechanism of DNA loading by the DNA repair helicase XPD DOI
    Diana Constantinescu-Aruxandei, Biljana Petrovic-Stojanovska, Carlos Penedo, Malcolm F White and James H Naismith, Nucleic Acids Research, 44, 6 , p. 2806-2815 (2016)
  10. Binding dynamics of a monomeric SSB protein to DNA DOI
    Michael John Morten, Jose Ramon Peregrina, Maria Figueira-Gonzalez, Katrin Ackermann, Bela Ernest Bode, Malcolm F White and Carlos Penedo, Nucleic Acids Research, 43, 22 , p. 10907-10924 (2015)
Last updated: 17 Mar 2016 at 20:48